Journal: Ecotoxicology and environmental safety

Article Title: Plumbagin, a natural derivative of 1,4-naphthoquinone, induces cyclopic phenomenon via increased apoptosis and ROS generation in the early stage of zebrafish embryos.

doi: 10.1016/j.ecoenv.2025.118535

Figure Lengend Snippet: Fig. 5. Neurodevelopmental inhibition by plumbagin (PL) in zebrafish embryos compared to positive controls. (a) Fluorescence images of three defined brain regions in transgenic zebrafish Tg(HuC:EGFP) embryos. The regions are outlined with dotted lines (fb, forebrain, blue; mb, midbrain, yellow; hb, hindbrain, orange). (b, c) Representative dorsal and ventral images of the head region in zebrafish embryos exhibiting cyclopia at 48, 72, and 96 h post-fertilization (hpf). The red triangle indicates the connection of optic nerves. Scale bar, 200 µm. EtOH, ethanol; Fsk, Forskolin; Cyc, cyclopamine.

Article Snippet: The stained embryos were mounted in 3 % methylcellulose, and ten embryos were randomly selected to photograph using a BX53 upright microscope with a DP80 color camera under a fluorescence light source (U-HGLGPS, Olympus Life Science Solutions, Waltham, MA, USA).

Techniques: Inhibition, Fluorescence, Transgenic Assay